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KMID : 1101420170490020135
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Korean Journal of Clinical Laboratory Science
2017 Volume.49 No. 2 p.135 ~ p.140
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Development of a Method to Detect Cattle Material from Processed Meat Products Using a Polymerase Chain Reaction
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Kwon Young-Chul
Hah Do-Yun
Heo Yun-Wi
Kim Tae-Kyu
Choi Yu-Jeong
Jo Dae-Hoon
Nam Sang-Yun
Son Byeong-Guk
Hwang Bo-Won
Yang Byoung-Seon
Kim Eui-kyung
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Abstract
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Polymerase chain reaction (PCR) was used to detect cattle material from processed meat products. Seventy-eight different commercial processed meat products were purchased from several big food marts. Among them, 17 products contained cattle material (10 samples contained only cattle, 5 samples mixed with cattle and porcine, 2 samples mixed with cattle, porcine and chicken). The genomic DNA was extracted directly from the processed meat products, and strain-specific primer targeting the 16S ribosomal RNA mitochondrial gene was used. All PCR products were cloned into the pGEM-T easy vector and sequenced. Consequently, the PCR products were amplified from 10 processed meat products, which contained only cattle material in our conditions. Furthermore, PCR reactions showed the same results at mixed samples. The DNA sequence obtained from pGEM-T easy/PCR products showed more than 95% identity with Bos taurus 16S rRNA gene using homology analysis. In conclusion, we suggest that the method using PCR, as performed in this study, could be useful in detecting cattle material in processed meat products. Moreover, our system could be applicable in inspection procedures to improve the verification of correct labeling for import and export processed meat products.
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KEYWORD
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16s rRNA, Polymerase chain reaction, Processed meat products, Inspection programs
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